| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lawsonia intracellularis | GTTGGGGATAGTTGCACGTG | CCTCTGAGGTTGACTTTGAGC | 59.20 | 58.58 | 214 | 173 |
100.00%
|
100.00%
|
| Primer 2 | Lawsonia intracellularis | AGTTGGGGATAGTTGCACGTG | CCTCTGAGGTTGACTTTGAGC | 60.61 | 58.58 | 215 | 173 |
100.00%
|
100.00%
|
| Primer 3 | Lawsonia intracellularis | ACAGAGCTTGACACCAGAGG | TTGCATCGCCTCCTGACTG | 59.32 | 60.08 | 252 | 163 |
100.00%
|
100.00%
|
| Primer 4 | Lawsonia intracellularis | GCTTTCAGCCACAGCAATGG | TGTCCAAGTTGGCCAATGG | 60.39 | 58.26 | 217 | 156 |
100.00%
|
100.00%
|
| Primer 5 | Lawsonia intracellularis | CTACCTTGCCCAACAGGAGG | ACCCATAACTGCTGGCTCTG | 60.04 | 59.75 | 252 | 152 |
100.00%
|
100.00%
|
| Primer 6 | Lawsonia intracellularis | TGGGGATAGTTGCACGTGT | CCTCTGAGGTTGACTTTGAGC | 58.94 | 58.58 | 212 | 148 |
100.00%
|
100.00%
|
| Primer 7 | Lawsonia intracellularis | TTGGGGATAGTTGCACGTGT | CCTCTGAGGTTGACTTTGAGC | 59.60 | 58.58 | 213 | 148 |
100.00%
|
100.00%
|
| Primer 8 | Lawsonia intracellularis | GCAGCATCGACAAAGTGGC | TCCCAGACTTCAAGGCCAAC | 60.15 | 59.89 | 190 | 143 |
100.00%
|
100.00%
|
| Primer 9 | Lawsonia intracellularis | GCAGCATCGACAAAGTGGC | GCCACCAACATCAGGGGAT | 60.15 | 59.69 | 153 | 141 |
100.00%
|
100.00%
|
| Primer 10 | Lawsonia intracellularis | TCTGAAGCCCTTGCCATGG | GCAAGCTGAGCGAACACTTC | 60.00 | 60.11 | 189 | 140 |
100.00%
|
100.00%
|